ISO 04979-2023.docx

INTERNATIONA1.STANDARDISO4979editionFirst2O23-O6Waterqua1.ityAquatictoxicitytestbasedonrootre-growthin1.emnaminorQUHitedeeauEssaidetoxicitaquatiquebassurIarepoussedesracineschez1.emnaminorCOPYRIGHTPROTECTEDDOCUMENT©ISO2023IUirhM*hedbdi1.iUedotherwiseupdhi.o啪InPSd1.Mc;GeatrOn0*tuuc11Dnt10tU%qn0*即b11o即PcXHiWKmt1.onmaytheinternetoranintranet,withoutpriorwrittenpermission.PermissioncanberequestedfromeitherISOattheaddressbe1.oworISO'smemberbodyinthecountr)oftherequester.f),WV>fifiU81.andonnet8CH-1214Vernier,GenevaPhone：M1.22749O1.11觥ftte:丽丽BQrgPub1.ishedinSwitzer1.andContentsForewordivIntroductionv2 Scope13 Normativereferences14 Termsanddefinitions16 Testorganisms3Growthmedium36.1 Preparationofstockso1.ution37 6.2Storageandcu1.tivation48 VPParatUS4,Experimenta1.methods58.1 1PrCPara口OnofIDedIUt1.)58.2 Preparationoftoxicantso1.utionandtestdi1.utions68.2.1 3,doiiwtUftistes1.concen1.ratians68.3 Contro1.68.4 T*rDsforOftestOrgan】SIDS.69 1."es5Oneffects79.1 Referencechcmica1.s792StatISt1.CS/Expressionofresu1.ts810.1Testresu1.tsB1110.2Expressionofresu1.ts8Testreport8Annex(informative)Rootexcisionandre-growth1.engthmeasurement10Annex(informativc)test12Bib1.iography一.一.14ForewordISO(theInternationa1.OrganizationforStandardization)isawor1.dwidefederationofnationa1.standardsttiugh(ISOmrtm匕0)bMdmi)rwcekdHPfff1.QbbGgbodyI1tf1.nmtiJMiai6sndrifc3owhtd1.ysQcchit2committeehasbeenestab1.ishedhastherighttoberepresentedonthatcommittee.Internationa1.organizations,governmenta1.andnon-governmenta1.,in1.iaisonwithISO,a1.sotakepartinthework.ISOco1.1.aboratesc1.ose1.ywiththee1.ectrotechnica1.standardization.Internationa1.E1.ectrotechnica1.Commission(IEC)ona1.1.mattersofTheproceduresusedtodeve1.opthisdocumentandthoseintendedforitsfurthermaintenanceare蜩喇搬of!SO1.E(tetheeditoria1.ru1.esoftheISO/IECDirectives.Part2(secwww.iso.org/direc1.ives).AnytradeusedinthisR«0nrights.dteE1.n殷的招舞ns副¥神吸融浒用qH1doc业我棉曲S双邯服.subjectofanypatentrightsidentifiedduringthedeve1.opmen13th»dOa向的twi1.1.beintheIntroductionand/orontheISO1.istofpatentdec1.arationsreceived(seewww.iso.org/patents).constitutenameendorsement,documentisinformationgivenfortheconvenienceofusersanddoesnotForanexp1.anationofthevo1.untarynatureofstandards,themeaningofISOspecifictermsand觐牌U那dOwarWSFMO)assE麻皿噩inwef像际R裂沁nBar池然MdeadPaes地w¼w.iso.oiso/forewoId.htm).早腋gicd带出*6脏WaSpreparedbyTechnica1.CommitteeISO/TC147.Waterqua1.itytSubcommitteeSC5,nyfeedbackorquestionsonthisdocumentshou1.dbedirectedtotheuser,snationa1.standardsbody.Acomp1.ete1.istingofthesebodiescanbefoundatwww.iso.org/members.1.Hm1.Introductionsevera1.standard20079:2005,U.S.haveEnvironmenta1.Protectionintemationa1.StandardizationEconomicexposuredurationsofat1.east7dtodetecttoxicity.rootsbyprovidingStabi1.itysensitivctoenvironmenta1.stressorspaidthatIhcyrootsimportanteco1.ogica1.However,thetoecotoxico1.ogica1.significanceeco1.ogica1.1.ysignificantendpointre-eva1.uatedandWith1.engthstandardizedmethodsre-growthbioassaydiffersinsevera1.keyaspectsfromthreeinternationa1.1.yb) thetestvesse1.isa24-we1.1.ce1.1.p1.ate;d)rootstechniqueexcisedexcisingexposuretosubsequentmeasurementsnorequirementdeve1.opedpre-se1.ectArtificia1.severanceofprevious1.y.Uoweverraccordingintonatura1.settingssincerootabsdssiondistributcdtheirdispersionsurviva1.potentia1.1.ybetter(environment.Therefore,rapidendpointabscissionre-growthconsideredorgans,we1.1.knownnonethc1.essimportantwithoutanchorage,nutrientabsorptionandnonessentia1.inappropriate.managementwastewaterIoxicitymadescrecningtime1.ymannerintheoperationa1.unexpectcdofpo1.1.utioneventsincegrowthrate/biomassendpoints.minor,butitcana1.sobeapp1.iedtoother1.emnaspeciesandSpirode1.aspecieswithsomemodifications.©ISO2023-A1.1.rightsreservedv1.emnagibhaand1.minorhavebeenmostextensive1.yusedinphytotoxicitytestingandthereare一forexamp1.e,ISOmethodswhich-beenadoptedbymajorAgency,Organizationforagencies.CooperationandDeve1.opment.Testswithduckweedhavetypica1.1.yfavouredmeasurementsoffrond(e.g.theirnumber,biomass,area,carbonuptake,ch1.orophy1.1.content(3j.(4)andrequirestandardOntheotherhand,testsbasedonroote1.ongationaresomeofthemostwide1.yusedphytotoxicitymethodo1.ogiesforterrestria1.angiospermsbecauseoftheirsimp1.icityandrapidity.Despitereportsthatro1.esof1.minorarehigh1.y5U6j.pj.(8jrhasbeenandtop1.ay1.emnasinceitwasgenera1.1.yconsideredthatrootfragi1.itymadetheirhand1.ingformeasurementsdifficu1.tandthatitwasimpractica1.toobtainsufficientnumbersofindividua1.p1.antswithidentica1.root1.engthstoinitiatetests.wasshownbesensitive,preciseandoftherootendpointhasbeenincomparisonrootmoretraditiona1.frondgrowthorbiomassendpoints.Theproposedroo